ABSTRACT
PURPOSE: To characterize the anatomy of the fruit and leaf and the presence of phytocompounds. To evaluate the antitumor and antimicrobial activity of ethanolic extract of Garcinia mangostana L. (mangosteen) cultivated in southeastern Brazil. METHODS: Anatomical characterization and histochemical reactions were performed for structural identification and the presence of phytocompounds. Preparation of ethanolic extract of the fruit, leaf and resin of mangosteen. Culture B16-F10 melanoma cells for treatment with mangosteen ethanolic extract to determine cell viability by MTT and genotoxic effect by comet assay. Evaluation by antimicrobial activity against Staphylococcus aureus and Escherichia coli by agar diffusion test and by determination of Minimum Inhibitory Concentration (MIC). RESULTS: Our results showed many secretory canals in resin fruit and leaf; identifying lipids, starch, lignin and phenolic compounds. The leaf extract induced genotoxicity and apoptosis in B16-F10 cells, since the fragmentation of DNA in the comet assay. The ethanolic extract of mangosteen obtained in the resin, leaf and fruit showed antimicrobial activity against Staphylococcus aureus and Escherichia coli with a MIC at 0.1 mg/mL. CONCLUSION: In conclusion, we have demonstrated both antimicrobial and antitumor activity of ethanol extract of mangosteen emphasizing its therapeutic potential in infectious diseases and in cancer, such as melanoma. .
Subject(s)
Animals , Mice , Anti-Infective Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Garcinia mangostana/chemistry , Plant Extracts/pharmacology , Antineoplastic Agents, Phytogenic/isolation & purification , Brazil , Cell Line, Tumor , Comet Assay , Cell Survival/drug effects , Enzyme-Linked Immunosorbent Assay , Fruit/chemistry , Microbial Sensitivity Tests , Melanoma/drug therapy , Reproducibility of Results , Staphylococcus aureus/drug effects , Time FactorsABSTRACT
PURPOSE: To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil. METHODS: Preparation ethanolic extract of the fruit of Morinda citrifolia L. Culture of melanoma cells B16-F10 for treatment with ethanolic extract of Morinda citrifolia L. fruit to determine cell viability by MTT and determination temporal effect of ethanolic extract fruit on the cell growth B16-F10 for 8 days. Evaluation of antimicrobial activity of ethanolic extract fruit against Staphylococcus aureus and Escherichia coli by determination of Minimum Inhibitory Concentration (MIC). RESULTS: The ethanolic extract of Morinda citrifolia L. fruit (10mg/mL) decreased cellular activity and inhibited 45% the rate of cell proliferation of B16-F10 melanoma treated during period studied. The ethanolic extract of Morinda citrifolia L. fruit demonstrated antimicrobial activity inhibiting the growth of both microorganisms studied. Staphylococcus aureus was less resistant to ethanolic extract of Morinda citrifolia L. fruit than Escherichia coli, 1 mg/mL and 10 mg/mL, respectively. CONCLUSION: What these results indicate that the ethanolic extract of the fruit of Morinda citrifolia L. showed antitumor activity with inhibition of viability and growth of B16-F10 cells and also showed antibacterial activity as induced inhibition of growth of Staphylococcus aureus and Escherichia coli. .
Subject(s)
Animals , Mice , Anti-Infective Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Morinda/chemistry , Plant Extracts/pharmacology , Analysis of Variance , Anti-Infective Agents/isolation & purification , Antineoplastic Agents, Phytogenic/isolation & purification , Brazil , Cell Line, Tumor , Cell Survival/drug effects , Drug Screening Assays, Antitumor/methods , Enzyme-Linked Immunosorbent Assay , Ethanol , Escherichia coli/drug effects , Fruit/chemistry , Microbial Sensitivity Tests , Melanoma/drug therapy , Reproducibility of Results , Staphylococcus aureus/drug effects , Time FactorsABSTRACT
Utilizando a técnica de hidrodestilação, usando um adaptador Clevenger, foram extraídos óleos essenciais das espécies Pimenta dioica (folhas e frutos) e Syzygium aromaticum (botões florais, talos e folhas). A composição química dos óleos foi determinada através da analise CG-EM. Os teores de óleos essenciais variaram de 0,97 a 1,41 por cento e 2,30 a 15,40 por cento nas espécies Pimenta dioica e Syzygium aromaticum, respectivamente. O componente majoritário presente nessas espécies foi o eugenol, variando de 72,87 a 90,41 por cento. Syzygium aromaticum forneceu maior teor de óleo essencial rico em eugenol. Em quantidades menores foram também encontrados chavicol e? β-cariofileno.
Essential oils were extracted from the leaves and fruits of Pimenta dioica and leaves, stalks and floral buttons from Syziguim aromaticum by hydrodistillation using a Clevenger apparatus. The essential oil compositions were determined by CG-MS analyses. The yield varied from 0.97 to 1.41 percent and from 2.30 to 15.40 percent in the P. dioica and S. aromaticum, respectively. In both species the major component was the eugenol, varied from 72.87 to 90.41 percent, being richer the essential oil extracted from S. aromaticum. Chavicol and β-caryophyllene were identified in low percentage.